In this investigation, using Opoola differential Operator ($D^{m}(\mu,\beta,t)f(z)$), a new Integral Operator: $I_{t,\beta,\mu}^{m,\sigma}(f_{1},...,f_{n})(z): A^{n}\rightarrow A$ is defined in the unit disk, $U=\left\lbrace z\in C:\left|z\right|<1\right\rbrace$; and we investigated the Univalence conditions of this generalized Operator. Finally, a number of corollaries and remarks which show the extension of our results are presented.